📙 leaf rust is a wheat disease and the wheat varieties which having leaf rust genes are resistant to it. Lr26 is also a leaf rust resistant gene found in various wheat varieties, having size of 1000 bps. The wheat varieties studied in this research was collected from various locations of Pakistan and observed that those varieties among the collected having Lr26 gene were not only resistant to the disease but also high in yield production and good in other morphological characters.