🔖 Abstract A field study was carried out during a three-year period (2008 to 2010) in order to investigate on response of Coker 347 (flue-cured) Tobacco plant (Nicotiana tabacum L.) in vegetative growth stages to inoculation with Azotobacter chroococcum at various levels of Nitrogen (N) fertilization.Results revealed that the effect of year of experiment was significant on quantitative characteristics (except leaf length), N uptake and qualitative characteristics (except nicotine in priming). Application of N had significant effect on quantitative characteristics (except number and width of Leaf), N uptake and qualitative characteristics. Inoculation of roots with bacterium had significant effect on Cured leaf weight of cutter, yield, N uptake and qualitative characteristics. Furthermore In general, Azotobacter chroococcum is a suitable inoculant for tobacco cultivation and it could be a strategy to achieve sustainable agriculture.