📕 The laboratory study was conducted to evaluate the persistence of Spirotetramat 240 SC (w/v) in sandy loam, loamy sand, sandy clay and clay soils. T0 - Untreated Control T1 - Spirotetramat 240 SC (w/v) @ 0.05 mg/L T2 - Spirotetramat 240 SC (w/v) @ 1.0 mg/L Collected the composite soil sample from 0-15 cm depth 2 hours (0 day) after the application. Continued the sampling on 1 3, 5 and 7 days for Spirotetramat and its BYI 08330 enol metabolite. All the samples were analysed for spirotetramat content by a validated HPLC-UV method with an acceptable mean recovery of spirotetramat; 92% for Sandy loam, 93% for Loamy sand, 91% for Sandy clay and 90% for Clay soil. The limit of determination (LOQ) is 0.03 ppm for Spirotetramat and its BYI 08330 enol metabolite.